Indole-3-Carbinol Induces Apoptosis in Human Osteosarcoma MG-63 and U2OS Cells

Figure 5

Detection of apoptosis by TUNEL assay in MG-63 (a) and U2OS (b) cells after 24 h and 48 h of treatment with different concentration of I3C. DNA fragmentations were detected using TUNEL assay in MG-63 and U2OS cells. Blue fluorescence shows nuclei, whereas green fluorescence shows DNA fragmentations. Blue-stained nuclei images and the green-stained fragmented DNA images were merged. The results showed that I3C induced DNA fragmentations in a dose- and time-dependent manner in MG-63 and U2OS cells.

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