Development of a New Molecular Marker for the Resistance to Tomato Yellow Leaf Curl Virus

Figure 2

Genetic characterization of ACY marker. (a) Polyacrylamide gel and (b) agarose gel electrophoresis of 36 commercial tomato hybrids using a newly developed ACY marker and Ty-3 linked marker P6-25, respectively. (c) Predicted position of ACY on tomato chromosome 6. (d) Structure of Ty-3 gene with 12 exons and 11 introns. (e) 10bp indel predicted between S. pennellii and S. lycopersicum; ATG and TGA represent start codon and stop codon, respectively; R indicates S. pennellii allele and S indicates S. lycopersicum. (f) Graphical representation of fragment size and primers position for ACY marker and those of DNA sequencing; X and vertical line indicate indel position; dashed line with double arrows indicates ACY amplified products (132bp and 123 bp); and double arrows line shows amplified DNA fragment for sequencing (630bp). (g) DNA sequencing results confirming 10bp insertion in resistant genotype and 10bp deletion in susceptible one; nucleotides in Italic are forward and reverse primers sequence from ACY. M in (a) and (b) were 500bp and 2000bp DNA Ladder Marker, respectively. 1: Pinkebabe, 2: Qianxi, 3: Huangrong, 4: Jiaxina (74-112), 5: Manxina (73-47) Messina, 6: Futesi 72-152, 7: Mantian 2025, 8: Hi-tech1, 9: Hi-tech2, 10: YuyiliangJingjing, 11: Aomei No. 1, 12: Oudun, 13: AG112, 14: AG115, 15: AG158, 16: AG1330, 17: Fengman No. 7, 18: Fengman No. 4, 19: Dongfeng 601, 20: NongboFenba No 1510, 21: ZhonghuaLvbao, 22: Jinfan102, 23: Duoxi13-1, 24: Nongqing 12-7, 25: Jinpeng 703, 26: Tianbao 326, 27: Yabao, 28: Luola, 29: Beiying, 30: Qidali, 31: Fenshou (74-560) RZ F1, 32: AG208, 33: Mantian 2218, 34: Mantian 2199, 35: Jingfan 502 (Ty1, Ty3a), 36: Hongshuang Xi (4224; Asterisk () represents the recombinants varieties.