LincRNA-p21 Inhibits Cell Viability and Promotes Cell Apoptosis in Parkinson’s Disease through Activating -Synuclein Expression

Figure 2

The miR-1277-5p was sponged by lincRNA-p21. (a) LincRNA-p21 was mainly located in cytoplasm. (b, c) The expression of miR-1277-5p was downregulated significantly in PD mice and PD model cells, SH-SY5Y. (d) RIP was performed and coprecipitated RNA was utilized for PCR assay. IgG, negative control group; input, total RNA group; AGO2, AGO2 antibody group. (e) The sequence of wide-type lincRNA-p21 or mutated lincRNA-p21 was inserted into the 3′ UTR of renilla luciferase (hRluc). (f) The luciferase activity was decreased significantly after transfection of miR-1277-5p mimics in lincRNA-p21 WT group. The luciferase activity was increased obviously after transfection of miR-1277-5p inhibitor in lincRNA-p21 WT group. However, there was no difference in lincRNA-p21 Mut group. All data are presented as the mean ± SD (P < 0.01, P < 0.001); bar, SD.

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