Table 5: Antibacterial spectrum of lipopeptide mixtures produced by Pseudomonas strains.

MIC of lipopeptide mixtures (mg/ml)
DSS73MFAD21cMFAF88MFAH4aMFAO2PfA7b

Gram positiveB. subtilis LMG 28342------
E. faecalis V583------
L.  ivanovii Li4pVS2------
L. monocytogenes EGDe ATCC BAA-679------

Gram negativeA. hydrophila LMG 2844------
E. coli LMG 2092------
F. breve LMG 4011------
K. pneumoniae 050283------
P. aeruginosa LMG 1242------
P. aeruginosa UCBPP-PA14------
S. enterica J18------
L. bozemanii ATCC 332170.090.140.610.190.380.20
L. dumoffii ATCC 332790.090.140.300.190.380.20
L. feeleii ATCC 350720.090.140.150.190.040.20
L. longbeachae ATCC 334840.180.140.300.190.190.20
L. micdadei ATCC 332180.180.270.300.190.190.20
L. pneumophila ATCC 33155 (Sg 3)0.180.270.610.380.380.40
L. pneumophila ATCC 33215 (Sg 6)0.360.270.610.380.380.40
L. pneumophila ATCC 33216 (Sg5)0.180.270.300.380.190.40
L. pneumophila ATCC Baa74 130b0.360.270.610.760.380.40
L. pneumophila Corby (Sg1)0.180.270.610.380.380.40
L. pneumophila Lens CIP 108286 (Sg 1)0.360.540.610.760.380.40

-: no growth of inhibition was detected even with undiluted lipopeptide mixture (0.72 mg/ml for the DSS73 mixture, 1.08 mg/ml for the MFAD21c mixture, 0.76 mg/ml for the MFAH4a mixture, 0.76 mg/ml for the MFAO2 mixture, 1.6 mg/ml for the PfA7b mixture, and 1.22 mg/ml for the MFAF88 mixture).
Bacteria (106 CFU/ml) were incubated in BYE or BHI medium with two-fold dilutions of lipopeptide mixtures. Results correspond to the MIC after incubation for 24h or 96 h at 37°C depending on the tested strain and are the mean of three independent experiments.
Bacterial strains were obtained from various culture collections: ATCC: American Type Culture Collection, CIP: Collection Institut Pasteur, France, and LMG: BCCM/LMG Bacteria Collection, Ghent University, Belgium. The Corby strain was kindly provided by the Centre National de Référence des Légionelles (Lyon, France). Other strains were from the laboratory culture collection. Sg: serogroup.