Research Article
Highlighting the Potency of Biosurfactants Produced by Pseudomonas Strains as Anti-Legionella Agents
Table 6
Antibacterial spectrum of rhamnolipid mixtures produced by Pseudomonas strains.
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
-: no growth of inhibition was detected even with undiluted rhamnolipid mixture (660 µg/ml for the 8H mixture, 1610 µg/ml for the CHA extract, 105 µg/ml for the PA14 extract, 100 µg/ml for the 4014 extract, and 250 µg/ml for the commercial mixture). Bacteria (106 CFU/ml) were incubated in BYE or BHI medium with two-fold dilutions of rhamnolipid mixtures. Results correspond to the MIC after incubation for 24h or 96 h at 37°C depending on the tested strain and are the mean of three independent experiments. Bacterial strains were obtained from various culture collections: ATCC: American Type Culture Collection, CIP: Collection Institut Pasteur, France, and LMG: BCCM/LMG Bacteria Collection, Ghent University, Belgium. The Corby strain was kindly provided by the Centre National de Référence des Légionelles (Lyon, France). Other strains were from the laboratory culture collection. Sg: serogroup. |