DREAM Is Involved in the Genesis of Inflammation-Induced Prolabour Mediators in Human Myometrial and Amnion Cells

Figure 5

Effect of siDREAM on proinflammatory cytokines, chemokines, and adhesion molecules in primary amnion cells. Primary amnion cells were transfected with 50 nM siCONT or 50 nM siDREAM for 48 h and then treated with 100 pg/mL IL-1β for an additional 24 h ( patients). (a, c, e, g) IL-6, IL-8, MCP-1, and ICAM-1 mRNA expression were analysed by qRT-PCR. (b, d, f, h) The incubation media was assayed for concentration of IL-6, IL-8, MCP-1, and sICAM-1 by ELISA. For all data, fold change was calculated relative to IL-1β-stimulated siCONT-transfected cells. Data are displayed as mean ± SEM. versus IL-1β-stimulated siCONT-transfected cells (one-way ANOVA).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)