DREAM Is Involved in the Genesis of Inflammation-Induced Prolabour Mediators in Human Myometrial and Amnion Cells

Figure 6

Effect of siDREAM on the ECM degrading enzyme MMP-9 in primary amnion cells. Primary amnion cells were transfected with 50 nM siCONT or siDREAM for 48 h and then treated with 100 pg/mL IL-1β for an additional 24 h ( patients). (a) MMP-9 mRNA expression was analysed by qRT-PCR. (b) The incubation media was assessed for pro-MMP-9 proteolytic activity using gelatin zymography. Representative zymogram from one patient is shown. For all data, fold change was calculated relative to IL-1β-stimulated siCONT-transfected cells. Data are displayed as mean ± SEM. versus IL-1β-stimulated siCONT-transfected cells (one-way ANOVA).

(a)

(b)