DREAM Is Involved in the Genesis of Inflammation-Induced Prolabour Mediators in Human Myometrial and Amnion Cells

Figure 7

Effect of siDREAM on NF-κB RelA transcriptional activity. Primary myometrial cells were transfected with 300 ng/ml NF-κB RelA reporter construct for 6 h, transfected with 50 nM siCONT or siDREAM for 48 h, and then treated with (a) 100 pg/ml IL-1β, (b) 250 ng/ml fsl-1, (c) 1 μg/ml flagellin, or (d) 5 μg/ml poly(I:C) for an additional 24 h (-6 patients). Promoter activity was normalised to Renilla expression. Fold change was calculated relative to IL-1β-, fsl-1, and flagellin- or poly(I:C)-stimulated siCONT-transfected cells. Data are displayed as mean ± SEM. versus IL-1β-stimulated siCONT-transfected cells; versus fsl-1-stimulated siCONT-transfected cells; versus flagellin-stimulated siCONT-transfected cells; versus poly(I:C)-stimulated siCONT-transfected cells (one-way ANOVA).

(a)

(b)

(c)

(d)