Optimization of Subcritical Water Extraction (SWE) of Lipid and Eicosapentaenoic Acid (EPA) from Nannochloropsis gaditana

Table 2

Experimental design matrix and responses of SWE of Nannochloropsis gaditana.

Standard order	Run order	Temperature, A (°C)		Extraction time, B (min)		Biomass loading, C (g algae/L)		Lipid yield (wt% of total biomass)		EPA composition (wt% of total FAME)
		Process	Coded	Process	Coded	Process	Coded	Experimental	Predicted	Experimental	Predicted
6	1	250	+1	10	-1	100	+1	9.265	10.690	14.425	16.825
8	2	250	+1	20	+1	100	+1	13.880	13.238	13.939	16.825
11	3	215	0	6.59	-α	75	0	12.053	10.995	15.784	12.454
4	4	250	+1	20	+1	50	-1	15.560	14.213	13.615	12.554
2	5	250	+1	10	-1	50	-1	16.420	17.145	12.483	12.554
7	6	180	-1	20	+1	100	+1	9.880	8.790	7.4837	6.228
3	7	180	-1	20	+1	50	-1	8.870	9.765	4.065	5.185
12	8	215	0	23.41	+α	75	0	9.513	10.733	8.855	12.646
5	9	180	-1	10	-1	100	+1	7.535	6.242	5.868	6.228
14	10	215	0	15	0	117.04	+α	9.407	10.313	14.889	15.657
17	11	215	0	15	0	75	0	15.667	16.887	19.365	12.646
1	12	180	-1	10	-1	50	-1	12.010	12.697	4.783	5.185
10	13	273.9	+α	15	0	75	0	15.313	15.136	10.389	9.775
13	14	215	0	15	0	32.96	-α	17.175	16.528	11.287	10.173
15	15	215	0	15	0	75	0	17.860	16.887	14.135	12.646
9	16	156.1	-α	15	0	75	0	7.260	7.650	3.155	2.933
16	17	215	0	15	0	75	0	17.173	16.887	13.155	12.646