Research Article
Optimization of Subcritical Water Extraction (SWE) of Lipid and Eicosapentaenoic Acid (EPA) from Nannochloropsis gaditana
Table 4
Fatty acid methyl ester composition from Soxhlet (8 hours using n-hexane) and SWE extraction (Run 15 at 215°C, 15 mins, and 75 g algae/L).
| Fatty Acid Methyl Ester (FAME) | Structure | Fatty Acid Composition (wt of total FAME) | Soxhlet | SWE (Run 15) |
| Caprylic | C8:0 | 0.069 | 0 | Capric | C10:0 | 0.062 | 0 | Undecanoic | C11:0 | 0.848 | 0.737 | Lauric | C12:0 | 0.334 | 0.990 | Tridecanoic | C13:0 | 0.215 | 2.020 | Myristic | C14:0 | 2.578 | 5.491 | Pentadecanoic | C15:0 | 0.209 | 0.378 | Cis-10-Pentadecenoic | C15:1 | 0 | 0.284 | Palmitic | C16:0 | 18.031 | 29.269 | Palmitoleic | C16:1 | 22.849 | 35.581 | Margaric | C17:0 | 0.205 | 0.312 | Cis-10-Heptadecenoic | C17:1 | 0.164 | 0 | Stearic | C18:0 | 0.436 | 0.481 | Oleic | C18:1n9c | 3.566 | 3.386 | Linoleic | C18:2n6c | 2.315 | 1.914 | -Linolenic | C18:3n6 | 0.337 | 1.072 | -Linolenic | C18:3n3 | 4.006 | 0 | Cis-11,14-Eicosadienoic | C20:2 | 0.996 | 0 | Cis-11,14,17-Eicosatrienoic | C20:3n3 | 0.763 | 1.037 | Cis-8,11,14-Eicosatrienoic | C20:3n6 | 3.083 | 2.434 | Arachidonic | C20:4n6 | 0.514 | 0 | Eicosapentaenoic (EPA) | C20:5n3 | 28.103 | 14.135 | Erucic | C22:1n9 | 6.393 | 0.478 | Docosadienoic | C22:2 | 2.259 | 0 | Tricosanoic | C23:0 | 1.664 | 0 |
| Saturated Fatty Acid (SFA) (wt of total FAME) | 25.647 | 39.678 | Monounsaturated Fatty Acid (MUFA) (wt of total FAME) | 32.973 | 39.730 | Polyunsaturated Fatty Acid (PUFA) (wt of total FAME) | 42.376 | 20.592 | Omega-3 (wt of total FAME) | 32.873 | 15.172 | Omega-6 (wt of total FAME) | 6.249 | 5.420 | Lipid Yield (wt of total biomass) | 10.200 | 17.860 |
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