Research Article
Monitoring Anti-NS1 Antibodies in West Nile Virus-Infected and Vaccinated Horses
Table 2
Results of serological examinations in field samples from WNV infected and vaccinated horses.
| Condition | Subgroup | Nº of sera | Anti-NS1 | Anti VLP | Ratio | (by origin/ vaccine type) | (mean OD) | OD(VLP)/OD(NS1) | OD | Posit | OD | Posit | R<4 | R>4 | (mean+s.d.) | n (%) | (mean+s.d.) | n (%) | n (%) | n (%) |
| Field-acquired infection | A2 (France) | 22 | 0.75±0.46 | 14 (63.6) | 1.29±0.89 | 22 (100) | 20 (90.9) | 2 (9.1) | A3 (Italy) | 17 | 0.45±0.28 | 6 (35.3) | 1.42±0.59 | 17 (100) | 10 (58.8) | 7 (41.2) | A4 (Morocco) | 24 | 0.69±0.29 | 17 (70.1) | 1.52±0.66 | 24 (100) | 21 (87.5) | 3 (12.5) | A5 (Spain) | 52 | 0.97±0.62 | 40 (76.9) | 2.79±1.73 | 52 (100) | 41 (78.8) | 11 (20.0) | TOTAL (A2-A5) | 115 | 0.72±0.41 | 77 (66.9) | 1.75±0.97 | 115 (100) | 92 (80.0) | 23 (20.0) |
| Field vaccination | B2 (Equip) | 26 | 0.29±0.29 | 4 (15.4) | 1.16±0.52 | 25 (96.1) | 13 (50.0) | 13 (50.0) | B3 (Proteq x1) | 30 | 0.42±0.2 | 9 (30.0) | 0.72±0.27 | 27 (90) | 30 (100) | 0 (0) | B4 (Proteq x2) | 75 | 0.23±0.28 | 4 (5.3) | 1.75±0.57 | 75 (100) | 14 (18.6) | 61 (81.3) | B5 (Recombitek +WNVvac) | 19 | 0.13±0.10 | 0 (0) | 2.08±0.33 | 19 (100) | 0 (0) | 19 (100) | B6 (WNVvacB6) | 13 | 0.43±0.39 | 5 (38.4) | 2.50±0.26 | 13 (100) | 4 (30.7) | 9 (69.2) | TOTAL (B2+B3+B4+B5+B6) | 163 | 0.31±0.27 | 22 (13.4) | 1.24±0.47 | 162 (98.7) | 61 (37.4) | 102 (62.6) |
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e. ( ) Positives (Indirect ELISA): values above 0.5 OD units. |