Research Article

Monitoring Anti-NS1 Antibodies in West Nile Virus-Infected and Vaccinated Horses

Table 2

Results of serological examinations in field samples from WNV infected and vaccinated horses.

ConditionSubgroupNº  of seraAnti-NS1Anti VLPRatio
 (by origin/ vaccine type)(mean OD)OD(VLP)/OD(NS1)
OD PositOD PositR<4 R>4 
 (mean+s.d.) n (%) (mean+s.d.)n (%) n (%) n (%)

Field-acquired infectionA2
(France)
220.75±0.4614 (63.6)1.29±0.8922 (100)20 (90.9)2 (9.1)
A3
(Italy)
170.45±0.286 (35.3)1.42±0.5917 (100)10 (58.8)7 (41.2)
A4
(Morocco)
240.69±0.2917 (70.1)1.52±0.6624 (100)21 (87.5)3 (12.5)
A5
(Spain)
520.97±0.6240 (76.9)2.79±1.7352 (100)41 (78.8)11 (20.0)
TOTAL (A2-A5)1150.72±0.4177 (66.9)1.75±0.97115 (100)92 (80.0)23 (20.0)

Field vaccinationB2
(Equip)
260.29±0.294 (15.4)1.16±0.5225 (96.1)13 (50.0)13 (50.0)
B3
(Proteq x1)
300.42±0.29 (30.0)0.72±0.2727 (90)30 (100)0 (0)
B4
(Proteq x2)
750.23±0.284 (5.3)1.75±0.5775 (100)14 (18.6)61 (81.3)
B5
(Recombitek +WNVvac)
190.13±0.100 (0)2.08±0.3319 (100)0 (0)19 (100)
B6
(WNVvacB6)
130.43±0.395 (38.4)2.50±0.2613 (100)4 (30.7)9 (69.2)
TOTAL (B2+B3+B4+B5+B6)1630.31±0.2722 (13.4)1.24±0.47162 (98.7)61 (37.4)102 (62.6)

e. () Positives (Indirect ELISA): values above 0.5 OD units.