Ischemic Preconditioning Promotes Autophagy and Alleviates Renal Ischemia/Reperfusion Injury

Figure 3

Ischemic preconditioning (IPC) alleviated renal ischemia/reoxygenation (I/R) injury. Male Sprague-Dawley rats were subjected to sham operation, IPC only, or 40 min of ischemia followed by 2 h, 6 h, 12 h, or 24 h of reperfusion with or without prior IPC. (a) Serum creatinine (Scr) concentrations. (b) Representative images of renal histology and quantitative analysis of tubular injury. Hematoxylin-eosin (H&E) staining. Representative slides of each group; ×200 magnification. (c) Representative images and quantitative data of terminal deoxynucleotidyl transferase-mediated nick-end labeling- (TUNEL-) positive nuclei in the kidney sections of each group. TUNEL staining; ×200 magnification. (d) Representative immunohistochemical images of tubular lesion in each group. ×400 magnification. KIM-1, kidney injury molecule-1; NGAL, neutrophil gelatinase-associated lipocalin. The data are expressed as the mean ± SD. per group. versus the sham group. versus the I/R group at the corresponding time point. versus the I/R 24 h group.

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