Ischemic Preconditioning Promotes Autophagy and Alleviates Renal Ischemia/Reperfusion Injury

Figure 4

Hypoxic preconditioning (HPC) induced autophagy and reduced apoptosis in renal tubular cells. HK-2 cells were cultured under normal conditions or oxygen and glucose deprivation (OGD) for 15 h followed by reoxygenation in normal complete medium for 30 min, 1 h, 2 h, 6 h, and 12 h with or without prior HPC, which consisted of OGD for 6 h and subsequent reoxygenation for 2 h. ((a) and (b)) Representative western blot gel documents and summarized data showing the levels of cleaved caspase-3, LC3II, and Beclin-1 protein expression. (c) Determination and quantitative analysis of apoptotic cells by Annexin V-propidium iodide FACS analysis. (d) Renal tubular cell viability was evaluated by CCK-8 assay. The data are expressed as the mean ± SD. per group. versus the control group. versus the H/R 2 h group. .

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