Effect of Astragalus Polysaccharides on Cardiac Dysfunction in db/db Mice with Respect to Oxidant Stress

Figure 6

Effects of APS on protein levels of oxidation target genes in myocardium of db/db mice. Left ventricular tissues obtained from C57BJ/6J mice and db/db mice with or without APS treatment (n=6 samples per group) were prepared and subjected to Western blotting of oxidation target genes. Membranes were stripped and reprobed for GAPDH as a loading control. Protein content was measured respectively and normalized as a ratio relative to the normal control. (a)~(f) Protein levels of Gpx, SOD2, t-JNK, p-JNK, catalase, p-p38 MAPK, t-p38 MAPK, t-ERK, and p-ERK. (g) Representative autoradiographs of Western blots. Values were presented as mean ± standard error of the mean (SEM). P<0.05 versus C57BJ/6J control mice, and #P<0.05 versus db/db mice. Gpx: Glutathione Peroxidase; t/p-JNK: total/phosphorylated c-Jun N-terminal kinase; t/p-p38 MAPK: total/phosphorylated p38 Mitogen-activated protein kinase; t/p-ERK: total/phosphorylate extracellular signal-regulated kinase.

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