Anti-Inflammatory Effect of Geniposide on Osteoarthritis by Suppressing the Activation of p38 MAPK Signaling Pathway

Figure 1

Characterization of normal and OA chondrocytes and the biocompatibility of geniposide. The positive expression of collagen II was demonstrated by immunofluorescence staining (a) compared with OA chondrocytes (b). The light microscopy revealed morphology of the normal and OA chondrocytes cultured in plates (c, d), identifying that the cells harvested from rabbits were chondrocytes. Notably, OA chondrocytes (f) were shown with slight blue color compared with normal chondrocytes (e) by using toluidine blue staining. The immunohistochemistry staining of collagen II showed that the positive staining with brown was observed at cytoplasm of normal chondrocytes (g), while the expression of collagen II was decreased in OA chondrocytes (h). (i) The MTT assay showed that geniposide with 10–160 μg/mL did not exhibit a cytotoxic effect on OA chondrocytes.

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