Anti-Inflammatory Effect of Geniposide on Osteoarthritis by Suppressing the Activation of p38 MAPK Signaling Pathway

Figure 4

Assessment of inflammatory mediators among different groups in vivo. (a–d) The expression level of TNF-α, IL-1β, MMP-13, and NO in the synovial fluid of different groups treated with geniposide and SB203580 as well as the combination of these two drugs, respectively. (e) qRT-PCR analysis of IL-1β, TNF-α, and MMP-13 gene expression in the synovial fluid of the knee joint treated with geniposide, SB203580, and combination of these two drugs, respectively. The data of control were considered as 1. (f) Western blotting of IL-1β, TNF-α, MMP-13, p-p38 (phosphorylation of p38), and t-p38 (total p38) protein expression among four groups treated with blank (control), geniposide, SB203580, and combination of these two drugs, respectively. (g) Quantification of western blotting. Data is given as the mean ± SD; and .

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