Aqueous Extracts of Cordyceps kyushuensis Kob Induce Apoptosis to Exert Anticancer Activity

Figure 3

AECK induced apoptosis in U937 and K562 (a) U937 and K562 were exposed to 64 μg/mL AECK for 48 h, and then Hoechst 33258 staining was used to detect apoptosis. (b) To analyze the DNA fragmentation, the cells were treated with the indicated concentrations of AECK for 48 h, and DNA was extracted, resolved in 1.5% agarose gel, and then visualized using ethidium bromide, and 2 μg/ml cordycepin was used to be positive control; (c) U937 was disposed of in different concentrations of AECK and cordycepin. The extracts from cells were assayed for caspase-3 activity by using colorimetric assay. (d) Flow cytometry analysis of U937 after treatment by increasing concentration of cordycepin and AECK for indicated time period.

(a)

(b)

(c)

(d)