Deregulation of Hexokinase II Is Associated with Glycolysis, Autophagy, and the Epithelial-Mesenchymal Transition in Tongue Squamous Cell Carcinoma under Hypoxia

Figure 5

Effects of HKII knockdown on the ability of autophagy, metastasis, and EMT in TSCC cells. (a) The levels of Beclin-1 and LC3 were evaluated after transient transfection. (b) The quantitative Beclin-1 data were computed as a ratio to levels of β-actin. (c) Representative results of the migration and invasion ability of TSCC cells were evaluated after transfection. Representative pictures of ×100 magnification. (d) Quantitative analysis of migratory ability. (e) The invasive ability quantitative data. (f) E-cadherin, Vimentin, Snail, and Slug expression levels were assessed after 24 h transfection. (g) Quantitative Snail data presented as a ratio to the β-actin level. All results are presented as mean ± SD obtained from three independent experiments. , , and .

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