Figure 8: Effect of DBD on cytokine in heart. For IL-6, TNF-α, and IL-1β assay in heart, the mice heart samples were homogenized on ice in saline solution and then centrifuged at 2500 × g for 10 min at 4°C to get the supernatants. IL-6, IL-1β, and TNF-α levels in heart measured by ELISA kits. All values given are the mean ± SD. #P<0.05 and ##P<0.01 versus control group. P<0.05 and P<0.01 versus CTX- group.