Genotypic Characterization of Rickettsia bellii Reveals Distinct Lineages in the United States and South America

Table 2

Primer pairs used for amplification of rickettsial genes or intergenic regions in the present study.

Primer pair	Target	Forward primer (5′ to 3′)	Reverse primer (5′ to 3′)	Annealing temperature (°C)	Amplicon size (nt)	Reference
(1)	mppA-purC	GCAATTATCGGTCCGAATG	TTTCATTTATTTGTCTCAAAATTCA	45 to 55^1	160	[33]
(2)	nusG-rplK	CAGTTGCAATATTGGTAAAGCA	CAGCAGCTGGAATTATCAAGTT	45 to 55^1	270	[33]
(3)	murG-RC0563	GAAGAAAAGAAGGGCATAAGCTA	CAAGCTGAAAGTAAAAACATTCC	40 to 52^1	293	[33]
(4)	ntrY-rpsU	AGCTGCTGTTGCTAAAGTAAAAA	CAAGAAGCAGCAAGAAGACAGA	52 to 64^1	363	[33]
(5)	dksA-xerC	TCCCATAGGTAATTTAGGTGTTTC	TACTACCGCATATCCAATTAAAAA	45 to 55^1	416	[33]
(6)	spo0J-abcT1	AAAGATTTGGAAGAATTAGACTTGAT	TTTGCTTAAACCAACCATTTCA	45 to 55^1	259	[33]
(7)	fabZ-lpxD	TGTTAGGATCGATTTTAAGTACTCTATCT	TGGATTGGCATAGACAATCTATTA	45 to 55^1	195	[33]
(8)	RC1137-tlc-5	CGGGATAACGCCGAGTAATA	ATGCCGCTCTGAATTTGTTT	45 to 55^1	264	[33]
(9)	RC0230-RC0231	TGCACCCGCCTAAAACTAAC	ATGGTCGGCCGTAGAAAAA	45 to 55^1	232	[33]
(10)	groES-RC0970	CTTGCATCGGCTTTTCTTTT	AGCTTTGAGCTGATGGGCTA	45 to 55^1	215	[33]
(11)	rrf-pyrH	GAGCTTTCTCCATCTTTTCTTG	AAAGGGGAATATACGACAATTGAG	45 to 55^1	238	[33]
(12)	-	AGCTTGGAAGGCTGGAACTC	ATCCTTCTCCCTCCACCACT	45 to 55^1	148	[33]
(13)	pal-RC1201	TGCAAGCACACATAATGCAA	TCAAAATCGATTCCTCTTTTCC	45 to 55^1	216	[33]
(14)	atpA	ATCAAGCGTTGCACAGATAG	GGAAGTGCCGTAAGTGAACC	58	Unknown	[1]
(15)	rpmE-tRN	TTCCGGAAATGTAGTAAATCAATC	TCAGGTTATGAGCCTGACGA	54	144	[33]
(16)	RC1027-xthA2	GGTATGTAAATGAGCCTTATCAATACT	TCAGTAGTATAAGTAGCTCCTGCTGTC	54	351	[33]
(17)	gltA	GTCTACTGCTTCGTGTAGATCAAC	GGCTGACCTATAGAATATTTATAAGAC	54	408	[25]
(18)	coxA	ACAGCCGTTGATATGGCTA	CATATTCCAACCGGCAAAAG	58	Unknown	[1]
(19)	coxA	GGTGCTCCTGATATGGCATT	CATATTCCAGCCGGCAAAAG	58	Unknown	[1]
(20)	atpA	ATCAAGCGTTGCACAGATAG	CGACTTACCGAAATACCGAC	56	449	[1, 34]

Data not given in the original publication; ¹tested in gradient PCR.