Research Article
Genotypic Characterization of Rickettsia bellii Reveals Distinct Lineages in the United States and South America
Table 2
Primer pairs used for amplification of rickettsial genes or intergenic regions in the present study.
| Primer pair | Target | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | Annealing temperature (°C) | Amplicon size (nt) | Reference |
| (1) | mppA-purC | GCAATTATCGGTCCGAATG | TTTCATTTATTTGTCTCAAAATTCA | 45 to 551 | 160 | [33] | (2) | nusG-rplK | CAGTTGCAATATTGGTAAAGCA | CAGCAGCTGGAATTATCAAGTT | 45 to 551 | 270 | [33] | (3) | murG-RC0563 | GAAGAAAAGAAGGGCATAAGCTA | CAAGCTGAAAGTAAAAACATTCC | 40 to 521 | 293 | [33] | (4) | ntrY-rpsU | AGCTGCTGTTGCTAAAGTAAAAA | CAAGAAGCAGCAAGAAGACAGA | 52 to 641 | 363 | [33] | (5) | dksA-xerC | TCCCATAGGTAATTTAGGTGTTTC | TACTACCGCATATCCAATTAAAAA | 45 to 551 | 416 | [33] | (6) | spo0J-abcT1 | AAAGATTTGGAAGAATTAGACTTGAT | TTTGCTTAAACCAACCATTTCA | 45 to 551 | 259 | [33] | (7) | fabZ-lpxD | TGTTAGGATCGATTTTAAGTACTCTATCT | TGGATTGGCATAGACAATCTATTA | 45 to 551 | 195 | [33] | (8) | RC1137-tlc-5 | CGGGATAACGCCGAGTAATA | ATGCCGCTCTGAATTTGTTT | 45 to 551 | 264 | [33] | (9) | RC0230-RC0231 | TGCACCCGCCTAAAACTAAC | ATGGTCGGCCGTAGAAAAA | 45 to 551 | 232 | [33] | (10) | groES-RC0970 | CTTGCATCGGCTTTTCTTTT | AGCTTTGAGCTGATGGGCTA | 45 to 551 | 215 | [33] | (11) | rrf-pyrH | GAGCTTTCTCCATCTTTTCTTG | AAAGGGGAATATACGACAATTGAG | 45 to 551 | 238 | [33] | (12) | - | AGCTTGGAAGGCTGGAACTC | ATCCTTCTCCCTCCACCACT | 45 to 551 | 148 | [33] | (13) | pal-RC1201 | TGCAAGCACACATAATGCAA | TCAAAATCGATTCCTCTTTTCC | 45 to 551 | 216 | [33] | (14) | atpA | ATCAAGCGTTGCACAGATAG | GGAAGTGCCGTAAGTGAACC | 58 | Unknown | [1] | (15) | rpmE-tRN | TTCCGGAAATGTAGTAAATCAATC | TCAGGTTATGAGCCTGACGA | 54 | 144 | [33] | (16) | RC1027-xthA2 | GGTATGTAAATGAGCCTTATCAATACT | TCAGTAGTATAAGTAGCTCCTGCTGTC | 54 | 351 | [33] | (17) | gltA | GTCTACTGCTTCGTGTAGATCAAC | GGCTGACCTATAGAATATTTATAAGAC | 54 | 408 | [25] | (18) | coxA | ACAGCCGTTGATATGGCTA | CATATTCCAACCGGCAAAAG | 58 | Unknown | [1] | (19) | coxA | GGTGCTCCTGATATGGCATT | CATATTCCAGCCGGCAAAAG | 58 | Unknown | [1] | (20) | atpA | ATCAAGCGTTGCACAGATAG | CGACTTACCGAAATACCGAC | 56 | 449 | [1, 34] |
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Data not given in the original publication; 1tested in gradient PCR. |