Isolation of Extracellular Vesicles: General Methodologies and Latest Trends

Table 2

Distribution of original research papers on EV isolation.

Method	Number of papers	Rate (%) of papers
(1.1) Ultracentrifugation with modifications	172	66.6
(1.2) Ultracentrifugation	118	45.7
(1.3) Density gradient ultracentrifugation	30	11.6
(2.1) Ultrafiltration	14	5.4
(2.2) Hydrostatic dialysis	2	0.7
(2.3) Size-exclusive chromatography	8	3.1
(3.1) Precipitation with polymers (PEG)	68	26.4
(3.2) Precipitation with protamine	1	0.4
(3.3) Precipitation with acetate	1	0.4
(3.4) Precipitation of proteins with organic solvent PROSPR	3	1.2
(4) Two-phase isolation	2	0.8
(5.1) Immunoprecipitation	5	1.9
(5.2) Annexin A5 coated magnetic beads	1	0.4
(5.3) Column-based affinity method	1	0.4
(5.4) Paper-based immunoaffinity devices	1	0.4
(5.5) Lectin binding	3	1.2
(5.6) Heparin binding	1	0.4
(5.7) Tim4 affinity-based method	1	0.4
(5.8) Vn96 binding	2	0.8
(6) Microfluidic devices	9	3.5