Chlorogenic Acid Functions as a Novel Agonist of PPAR2 during the Differentiation of Mouse 3T3-L1 Preadipocytes

Figure 2

Comparative effect of chlorogenic acid (CGA) and rosiglitazone (RG) on the differentiation of mouse 3T3-L1 preadipocytes. (A) 3T3-L1 preadipocytes were cultured with or without CGA (20 μM) for 10 days; then cells were stained with ORO. RG was used as a positive control. GW9662 was used as a negative control group (GG). All images are shown at 200 × magnification. (a) Control group (CG); (b) RG group; (c) GG group; (d) CGA group; the arrows in a-d indicate the differentiation of preadipocytes; (B-C) effects of CGA on the expression of lipogenic pathway-related genes during the differentiation process of mouse 3T3-L1 preadipocytes. (B) Plin; (C) Srebp1. Data are shown as the means ± SD (n = 3). ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001.