Chlorogenic Acid Functions as a Novel Agonist of PPAR2 during the Differentiation of Mouse 3T3-L1 Preadipocytes

Figure 5

Chlorogenic acid (CGA) activates PPARγ2 during the differentiation process of mouse 3T3-L1 preadipocytes. (a) Mouse 3T3-L1 preadipocytes were treated with control (control group; CG), rosiglitazone (RG), GW9662 (GG), and CGA for 2, 4, 6, and 8 days, respectively. The samples were lysed and subjected to western blot analysis with indicated antibodies. Left: total cell lysate; Right: nucleus fraction. (b) The intensity of the band was quantified using densitometric imaging. Data are shown as the means ± SD (n = 3). ^∗P < 0.05, ^∗∗P < 0.01.

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