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BioMed Research International
Volume 2018, Article ID 8607647, 7 pages
https://doi.org/10.1155/2018/8607647
Research Article

Molecular Characterization of Antimicrobial Resistance in Escherichia coli from Rabbit Farms in Tai’an, China

College of Animal Science and Technology, Shandong Agricultural University, Tai’an 271000, China

Correspondence should be addressed to Weishan Chang; ten.haey@nahsiewgnahc and Shuhong Sun; moc.361@sjykfbyqj

Received 8 September 2017; Revised 13 December 2017; Accepted 28 January 2018; Published 26 February 2018

Academic Editor: Wen-Jun Li

Copyright © 2018 Xiaonan Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To investigate the prevalence and resistance against antimicrobials of Escherichia coli (E. coli) in Tai’an, March 2016, a total of 55 E. coli strains were isolated from 60 faecal samples of diarrheic rabbits collected from three rabbit farms in Tai’an. The E. coli isolates were assayed for antimicrobial susceptibility and prevalence of resistance genes and Class I integrons and genotyped using Multilocus Sequence Typing (MLST). All the E. coli isolates were sensitive to ceftazidime, ceftriaxone, imipenem, and amikacin, while 78.2% of the isolates showed resistance against tetracycline, and 65.5% were resistant against ampicillin. The most common resistance gene detected was , present in 98.2% of isolates, followed by (94.6%) and sul2 (58.2%). Class I integrons were detected in 17 out of the 55 (30.9%) E. coli strains. Seven kinds of gene cassette were detected: dfrA17 + aadA5, dfrA1 + catB3 + aacA4, aadA2 + LinF, dfrA1 + aadA1, aadA22, dfrA12 + orfF + addA2, and aadA16 + dfrA27 + arr-3. All the 55 E. coli strains were identified and classified as 13 sequence types (STs); ST302 (22/55, 40.0%) was the most prevalent type, followed by ST370 (12/55, 21.8%). This study showed that E. coli isolated from diarrheic farmed rabbits in the Tai’an area exhibit sometimes very frequent resistance to antimicrobials important to human medicine, which further highlights the need for reasonable use of antibiotics.