Research Article
Zerumbone-Loaded Nanostructured Lipid Carrier Induces Apoptosis of Canine Mammary Adenocarcinoma Cells
Table 1
Primer sequences for the characterization of the canine mammary gland tumor (CMT) adenocarcinoma cell.
| | Genes | Primer Sequence (5’ – 3’) | Annealing Temperature (°C) |
| Cytokeratin-8
(CK-8) | Forward:
AAGCAGCTTCTCCGCTCCTTCTA
Reverse:
CCAGCTTCAGTTTCTCCTAGCCCA | 50 |
| Hypoxanthine ribosyltransferase
(HPRT) | Forward:
TTA TCA GAC TGA AGA GCT ACT
Reverse:
TTA CCA GTG TCA ATT ATA TCT TCA ACA ATC | 60 |
| Estrogen Receptor
(ER) | Forward:
TCAGCTCTCGTCCTTCCTGCA
Reverse:
ACCAGACTCCATAATGGTAGCCTG | 56 |
| Progesterone Receptor
(PGR) | Forward:
AGGTGTACCAGCCGTACCTCAA
Reverse:
TTCGACCTCCAAGGACCATGCCAG | 56 |
| Vascular Endothelial Growth Factor Receptor
(VEGF) | Forward:
GCTCTCTTGGGTGCATTGGAGC
Reverse:
TAGGCCCTCATCATTACAGCAGC | 58 |
| | Human epidermal growth factor receptor 2 (HER2) | Forward:
ACCGGCACAGACATGAAGCTCC
Reverse:
AGCGGGTCTCCATTGTCCAGCA | 50 |
| Hypoxia-inducing Factor-1α
(HIF-1α) | Forward:
CGTTCCTTCGATCAGTTGTC
Reverse:
TCAGTGGTGGCAGTGGTAGT | 56 |
| B-cell lymphoma-2
(Bcl-2) | Forward:
ATGTGTGTGGAGAGCGTCAACC
Reverse:
TGAGCAGAGTCTTCAGAGACAGCC | 50 |
| Matrix Metallopeptidase-2
(MMP-2) | Forward:
CCACGTGACAAGCCCATGGGGCCCC
Reverse:
GCAGCCTAGCCAGTCGGATTTGATG | 60 |
| Erythropoietin Receptor
(EPOR) | Forward:
CCTGACGCTCTCCCTCATCC
Reverse:
GCCTTCAAACTCGCTCTCTGG | 65 |
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