Zerumbone-Loaded Nanostructured Lipid Carrier Induces Apoptosis of Canine Mammary Adenocarcinoma Cells

Table 3

Primer sequences for PCR analysis of apoptotic-related and reference genes.

Gene	Forward and reverse primers (5’ – 3’)	Annealing Temperature (°C)	Product length (bp)	Accession number
Bcl-2	Forward: TGG ATG ACT GAG TAC CTG AA; Reverse: GGC CTACTG ACT TCA CTT AT	56.00	206.00	AB116145
Bax	Forward: GGT TGT TGC CCT CCT CTA CT; Reverse: GTA AGC ACT CCA GCC ACA AA	60.00	219.00	AB080230
RPS-19	Forward: CCT TCC TCA AAA AGT CTG GG; Reverse: GTT CTC ATC GTA GGG AGC AAG	61.00	95.00	XM_533657
GAPDH	Forward: TGT CCC CAC CCC CAA TGT ATC; Reverse: CTC CGA TGC CTG CTT CAC TAC CTT	58.00	100.00	NM_001003142