The Involvement of Arginase and Nitric Oxide Synthase in Breast Cancer Development: Arginase and NO Synthase as Therapeutic Targets in Cancer

Figure 2

Arginase activity (▲) and protein quantity (■) curves after gel chromatography of serum proteins in healthy individuals, breast cancer patients, and patients after chemotherapy (stage II, , ). (a) Healthy, (b) breast cancer, and (c) chemotherapy. The enzyme obtained after several steps of purification (see Materials and Methods; 45 mg, 51 mg, and 52 mg protein, resp., for healthy individuals, breast cancer patients, and patients after chemotherapy) was applied to a Sephadex G-150 column (2 × 40 cm).

(a)

(b)

(c)