Using CRISPR/Cas9 to Knock out Amylase in Acinar Cells Decreases Pancreatitis-Induced Autophagy

Figure 3

Amylase deletion acinar cells. (a) Genomic DNA sequence of independent 4 clones in which Amy2 was deleted. Bases shown in red are indels (insertions and deletions). PAM sequence is shown in green. (b) Amylase-deficient AR42J western blotting results for 4 independent amylase-deficient AR42J clones. LC3-I is a precursor protein to the autophagy marker LC3-II. β-Actin was the loading control. (c) Wild-type AR42J was incubated with standard medium, 100 nM caerulein, or 100 nM rapamycin for 3 h, or in starvation medium for 1 h. LC3-II is an autophagy marker.

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