Using CRISPR/Cas9 to Knock out Amylase in Acinar Cells Decreases Pancreatitis-Induced Autophagy

Figure 4

Amylase deletion and autophagy. (a) Wild-type and Amy2-deficient clones were incubated in 100 nM rapamycin for 3 hr. Western blotting results. LC3-II is an autophagy marker. (b) Wild-type and Amy2-deficient AR42J cells were assayed by immunofluorescence and observed by confocal microscopy. Green is LC3; blue is Hoechst stain (c) Wild-type and AMY2-deficient clones were incubated in 100 nM cerulein for 3 h. Western blotting results.

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