Comprehensive Validation of Snapback Primer-Based Melting Curve Analysis to Detect Nucleotide Variation in the Codon 12 and 13 of KRAS Gene

Figure 1

Mutation Landscape of KRAS in TCGA (7,844 primary tumors across 32 cancer types) and MSK-IMPACT (10,366 metastatic cancers) cohort. (a) Onco-print of KRAS genetic alterations across 32 types of primary tumors in TCGA. Somatic mutations are the dominating type of genetic alteration. PAAD, COAD, and LUAD are the top 3 KRAS most mutant cancer types. Copy number amplification are frequently found in TGCT and OV. (b) Lollipop plot of the KRAS mutational hotspot in the TCGA PAAD, COAD, and LUAD cohort. The KRAS mutation is highly prevalent in PAAD. Codons 12 and 13 are the most prevailing mutation hotspot. COAD showed a relatively high KRAS mutation pattern diversity. (c) Onco-print of KRAS genetic alterations in the MSKCC-IMPACT cohort. Somatic mutations are the most common type of genetic alteration across metastatic cancers originated from 33 types of tissue primaries. Both the frequency and the diversity of the mutations are higher than those in the TCGA cohort.

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