Comprehensive Validation of Snapback Primer-Based Melting Curve Analysis to Detect Nucleotide Variation in the Codon 12 and 13 of KRAS Gene

Table 2

Distribution of KRAS mutations detected by Snapback Primer-based HRM, Sanger sequencing, and T-A Cloning Sequencing in 100 CRC tissues.

Methods	G12D	G12V	G12C	G12A	G13D	Total
Snapback primer-based HRM	14	10	1	1	9	35
Direct sequencing	12	8	1	0	7	28
T-A Cloning Sequencing	2	2	0	1	2	7