Resveratrol Inhibits the TGF-β1-Induced Proliferation of Cardiac Fibroblasts and Collagen Secretion by Downregulating miR-17 in Rat

Figure 3

Effect of resveratrol on miRNAs and Smad7 mRNA/protein expression in CFs. (a) Analysis of differential miRNA expression in CFs by qPCR. (b) miR-17 expression in CFs after miR-17 overexpression/silencing. (c) Smad7 mRNA expression in CFs after miR-17 overexpression/silencing. (d) Smad7 protein expression in CFs after miR-17 overexpression/silencing. P<0.001 versus the control group; ###P<0.001 versus the TGF-β1 group; &&&P<0.001 and &P<0.05 versus the TGF-β1+resveratrol group; $$$P<0.001 versus the TGF-β1+NC group; %%%P<0.001 versus the TGF-β1+miR-17 mimics group; @@@P<0.001 versus the TGF-β1+ miR-17 inhibitor group (n=3). C: Control; T: TGF-β1; T+R: TGF-β1+RSV; T+NC: TGF-β1+negative control; T+m: TGF-β1+miR-17 mimics; T+i: TGF-β1+miR-17 inhibitor; T+m+R: TGF-β1+miR-17 mimics+RSV; T+i+R: TGF-β1+miR-17 inhibitor+RSV.

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