Resveratrol Inhibits the TGF-β1-Induced Proliferation of Cardiac Fibroblasts and Collagen Secretion by Downregulating miR-17 in Rat

Figure 4

The effect of miR-17 and Smad7 on the proliferation of cardiac fibroblasts and collagen secretion after TGF-β1 treatment. (a) Effect of transfection of Smad7 overexpression plasmid on Smad7 protein levels in each group. A: control; B: pcDNA 3.1(+) vector; C: pcDNA 3.1(+)-Smad7. (b) Representative images of EdU incorporation assays. C: control; T: TGF-β1; T+R: TGF-β1+RSV; T+NC: TGF-β1+NC; T+i: TGF-β1+miR-17 inhibitor; T+i+R: TGF-β1+miR-17 inhibitor+RSV; T+p: TGF-β1+pcDNA.3.1(+) vector; T+S: TGF-β1+pcDNA.3.1(+)-Smad7; T+S+R: TGF-β1+pcDNA.3.1(+)-Smad7+RSV. (c) The CF proliferation rate as determined by CCK-8 assay. (d) The relative ratio of EdU-positive cells. (e) The secretion of hydroxyproline as measured using the hydroxyproline assay kit. P<0.001 versus the control group; %%%P<0.001 versus the pcDNA.3.1(+) vector group; ##P<0.01 and ###P<0.001 versus the TGF-β1 group; &P<0.05, &&P<0.01, and &&&P<0.001 versus the TGF-β1+RSV group; $$P<0.01 and $$$P<0.001 versus the TGF-β1+NC group; @@P<0.01 and @@@P<0.001 versus the TGF-β1+miR-17 inhibitor group; △△△P<0.001 versus the TGF-β1+pcDNA.3.1(+) vector group; ☆☆P<0.01 and ☆☆☆P<0.001 versus the TGF-β1+pcDNA.3.1(+)-Smad7 group (n=3).