Research Article
Resveratrol Inhibits the TGF-β1-Induced Proliferation of Cardiac Fibroblasts and Collagen Secretion by Downregulating miR-17 in Rat
Table 1
Primer sequences of 5S and miRNAs for real time-polymerase chain reaction.
| Gene | primer | Sequence |
| 5S | Forward | TGGGTTCATTTCTGGGTCTT | 5S | Reverse | GGATGGGAGACCGCCTGGGAATAC | 5S | Stem-loop | GAGTAGACCAATGGGTTCATTTCTGGGTCTTATTCTATTCCATTGGTCTACTCAAAGCCTA | miR-17 | Forward | GTACCAAAGTGCTTACAGTGC | miR-17 | Stem-loop | GAGTAGACCAATGGGTTCATTTCTGGGTCTTATTCTATTCCATTGGTCTACTCCTACCT | miR-181a | Forward | GTCTAACATTCAACGCTGTCG | miR-181a | Stem-loop | GAGTAGACCAATGGGTTCATTTCTGGGTCTTATTCTATTCCATTGGTCTACTCACTCAC | miR-34a | Forward | GTCTTGGCAGTGTCTTAGCT | miR-34a | Stem-loop | GAGTAGACCAATGGGTTCATTTCTGGGTCTTATTCTATTCCATTGGTCTACTCACAACC |
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The reverse primers of all microRNAs were the same as those of 5S.
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