Divergent Evolutional Mode and Purifying Selection of the KIT Gene in European and Asian Domestic Pig Breeds

Table 1

Primer sets used in this study.

Primer Name	Sequence (5’-3’)	Product size (bp)	TM (°C)
KIT_E1F	GGAACGTGGAAAGGAGCT	205	66
KIT_E1R	CAGCCACCTCTTCTGACAC
KIT_E2F	ACCCTTGCACCATAAATAGC	498	59
KIT_E2R	GACTCAGCCATCTATATGTC
KIT_E3F	TCAGATTCGTTAACCACTGC	447	64
KIT_E3R	TGGCATATCCGCTGATCTA
KIT_E4F	CGAAAGGCCATACAGTGAG	362	50
KIT_E4R	TGTATGAAAGCAACTATACC
KIT_E5F	TCCCCCCGGGACTGCAGAGATTTGGGAATTATG	548	59
KIT_E5R	CGCGGATCCTCAACCTACTACTTCTAAGGTGG
KIT_E6F	GAAGACATAAGATGGTGATA	385	53
KIT_E6R	CTAATATCCATGAGGACGCAGG
KIT_E7F	CCAAAGCAGGCGTGTATTT	344	55
KIT_E7R	GGTCAAAATTATCATGAAGCAG
KIT_E8F	GTCATCTTCAGCCTCAAGAAAG	313	54
KIT_E8R	TCTGAAAACATATTTGAAATTC
KIT_E9F	GGCAAATAATTTTTCTTCTAG	293	54
KIT_E9R	CAGGCAGAGCCTAAACATCC
KIT_E10-11F	CTTGACTCCTGCCATGATG	536	64
KIT_E10-11R	CTAGAACAAAGGAAGCTACCG
KIT_E12-13F	GTCCCTGATTCCTTTATTGG	486	60
KIT_E12-13R	GCTTATCATCAAGGATGGTC
KIT_E14F	GCATGTGAATGGCCGTGATC	322	50
KIT_E14R	ATCAGCCTTGATTGCAAACC
KIT_E15-16F	CAAAGTCAGCCTCTTATGTGA	910	52
KIT_E15-16R	CTACGGCTCTAAAATGCTCC
KIT_E17F	TGGCACCATATAACATAGGC	325	60
KIT_E17R	GGTGTGCATTATGAAACTCAC
KIT_E18-19F	GCAGCAGGAGCAGTATCTAC	482	63
KIT_E18-19R	AACCTTCAACATCTGGGTTC
KIT_E20F	GGTGTACACCCAAAGACAG	217	63
KIT_E20R	CTGTTCAAGGCGTTCCAAGC
KIT_E21F	ACTTGCGATTCTGGACCTGC	271	63
KIT_E21R	GAATGGCAGTAGGTCGGTGC