The Antiosteoporosis Effects of Zhuanggu Guanjie Pill In Vitro and In Vivo

Figure 2

ZGGJ inhibits bone resorption and MMP9 in vitro. (a) ZGGJ had no significant effect on ALP activity in vitro. (b) ZGGJ reduced osteoclast TRACP activity in vitro. (c) MMP9 detected by gelatin zymography. (d) Representative photomicrographs (×9) of bone slices and numbers of resorption pits. (e) Representative photomicrograph (×40) of bone slices and total areas of resorption pits. Con, control group with cells cultured in medium without M-CSF or RANKL; Mod, model group with cells cultured in the presence of 15 ng/mL M-CSF and 30 ng/mL RANKL; ZGGJ, groups treated with various doses of ZGGJ. ^#p < 0.05 versus Con; p < 0.05 versus Mod.

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