Inoculation with Efficient Nitrogen Fixing and Indoleacetic Acid Producing Bacterial Microsymbiont Enhance Tolerance of the Model Legume Medicago truncatula to Iron Deficiency

Table 1

Results of three-way analysis of the effect of strain (S), genotypes (G), and treatment (T) and their interaction (STG) on shoots weight (ShW: g plant⁻¹), roots weight (RW: g plant⁻¹), roots length (RL, cm plant⁻¹), content of iron in shoots (Fe Sh: mg g⁻¹ plant) and roots (Fe R: mg g⁻¹ plant), chlorophyll (Chl: mg g⁻¹ FW), and antioxidant enzyme activities in shoots (SOD Sh (USOD 10³  μg⁻¹ protein), CAT Sh (mM H₂O₂ min⁻¹ mg⁻¹ protein), and POX Sh (mM H₂O₂ min⁻¹ mg⁻¹ protein)) and roots (SOD R: USOD 10³  μg⁻¹ protein, CAT R (mM H₂O₂ min⁻¹ mg⁻¹ protein), and POX R (mM H₂O₂ min⁻¹ mg⁻¹ protein)) in Medicago truncatula genotypes.

	Sh W	RW	LR	Fe Sh	Fe R	Chl	SOD Sh	SOD R	CAT Sh	CAT R	POX Sh	POX R
S	48,4	1588	33,2	247,4	698,8	929	173	133,6	34,7	104,4	7,47	19,81
G	785	2946,3	715,9	171,9	783,3	174	6,25	51,52	89,68	167,	93,58	88,78
T	135,2	2,68	122,6	194,4	215,2	315	63,9	5,58	81,37	5,29	0,52	49,26
SG	108	260	48	390	515	52	15	38	72	31	32	100
ST	10	17	20	21	225	52,6	66	12,8	40	29,6	9,36	8,6
GT	14,25	26,7	78	26,3	527,4	11	9,25	7,24	30,69	19,12	4,59	18,8
SGT	3,7	7,54	13,4	12,8	314,2	12,8	3,69	6,31	7,74	44,22	14,65	88,5

Numbers represent F-value: P<0.0001, P<0.001, and P<0.01.
ns: nonsignificant.