Developmental Programming of Capuchin Monkey Adrenal Dysfunction by Gestational Chronodisruption

Figure 2

Effects of exposure to constant light during the last third of gestation on adrenal weight (mg; mean ± SEM), histology (a and b, magnification 10X; the bar indicates 400 m); adrenal expression of StAR, 3-HSD, and Mt1 mRNA (c, d, and e, respectively), and StAR and 3-HSD protein (f-h) in 10-month-old infants. Representative slot-blot panel (g) for StAR, 3-HSD, and the housekeeping -ACTIN protein. Black bars, 10-month-old infants from mothers maintained in 14:10 light:dark cycle (control, n=4). White bars, 10-month-old infants from mothers maintained with lights continuously on from about 100 days of gestation to delivery (LL, n=4). Different to control (P<0.05; Student’s t-test).