BioMed Research International

Review Article

Barriers and Advances in Kidney Preservation

Table 1

Characteristics of current kidney preservation solutions and machine perfusion.


Solutions	K⁺	Na⁺	Buffer	pH	Impermeant	Adenosine	Anti-oxidant	Colloid (g/L)
Solutions	(mM)	(mM)	Buffer	pH	Impermeant	(mM)	Anti-oxidant	Colloid (g/L)

Flush and Static cold storage

Blood	4.25	139	HCO3-	7.4	+	0	+	Albumine (50 g/L)

HTK (Custodiol®)	10	15	Histidine	7.2	+	5	-	-

UW (Viaspan®) (Bridge to life®)	100	28.5	(K)H2PO4 HEPES	7.4	+	5	Glutathion	HES (50 g/L)

Celsior®	15	100	HEPES	7.3	+	0	Glutathion	-

IGL-1®	30	125	(K)H2PO4	7.3	+	5	Glutathion Allopurinol	PEG 35kDa (1g/L)

Lifor®	16	98	Phosphates	7.07	+	?	?	?

Polysol®	15	120	(K)H2PO4 HEPES Histidine	7.4	+	5	Glutathione Acid ascorbic	PEG 35kDa (20g/L)

SCOT 15®	5	118	HCO3-	7.4	+	0	-	PEG 20 kDa (15g/L)

Flush solutions

Carolina RS®	5	115	(K)H2PO4	6,5	+	1	Glutathion Allopurinol	HES (50 g/L)

Dynamic preservation solution (for hypothermic perfusion machine)

KPS-1®	25	97.5	(K)H2PO4 HEPES	7.4	+	5	Glutathion	HES (50 g/L)

PERF-GEN®	25	100	(K)H2PO4 HEPES	7.4	+	5	Glutathion	HES (50 g/L)

MPS®	25	100	(K)H2PO4 HEPES	7.4	+	5	Glutathion	HES (50 g/L)

Kidney hypothermic perfusion machines

Machine	Solution type		Pulsatile perfusion		Temperature		Oxygen supply (100)

LifePort®	KPS-1® MPS®		-		4°C		No

WAVES®	PERF-GEN®		+		4°C		100%

Kidney Assist-Transport®	KPS-1® MPS®		-		4°C		100%

HTK (Custodiol®, Dr Franz Köhler Chemie GMBH, Alsbach-Hähnlein, Germany); UW (University of Wisconsin, Alumni Research Foundation, Madison, WI, USA); Celsior® (Genzyme Corporation, Cambridge, MA, USA); IGL-1® (Institut Georges Lopez, Civrieux d’Azergues, France); Lifor™ (Lifeblood Medical, Freehold, NJ, USA); Polysol® (Doorzand Medical Innovations B.V., Amsterdam, The Netherlands); SCOT15® (MacoPharma, Tourcoing, France); Carolina RS® (Carolina Rinse Solution, University of North Carolina, Chapel, USA); KPS-1® (Organ Recovery Sytems, Chicago, USA and Brussels, Belgium); MPS® (Belzer MPS® UW Machine Perfusion Solution, Bridge to life; Columbia, USA); PERF-GEN® (Institut Georges Lopez, Civrieux d’Azergues, France); LifePort® (Organ Recovery Sytems, Chicago, USA and Brussels, Belgium); WAVES® (Institut Georges Lopez, Civrieux d’Azergues, France); Kidney Assist-Transport® (Organ Assist B.V, Groningen, The Netherlands); HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid); HES (hydroxyethyl starch); PEG (Polyethyleneglycol).