Ecotoxicological and Interactive Effects of Copper and Chromium on Physiochemical, Ultrastructural, and Molecular Profiling in Brassica napus L.

Table 2

Effects of copper (Cu) and chromium (Cr) on MDA (nmol g⁻¹ FW), hydrogen peroxide (H₂O₂) (μmol g⁻¹ FW), superoxide radical () (nmol min⁻¹ g⁻¹ FW), and hydroxyl radical (μmol g⁻¹ FW) in the leaves of two Brassica napus cultivars under different metal treatments.

Cultivar	Treatment	Leaf MDA	Leaf	Leaf H2O2	Leaf
ZS 758		6.02 ± 0.21	1.88 ± 0.10	1.57 ± 0.06	0.013 ± 0.0035
	Cu 200 μM	11.20 ± 0.71	10.41 ± 0.88	51.97 ± 2.06	0.526 ± 0.0075
	Cr 200 μM	17.81 ± 1.18	19.43 ± 0.52	71.05 ± 1.80	0.770 ± 0.0280
	Cu 200 μM + Cr 200 μM	25.95 ± 1.08	25.92 ± 1.10	96.19 ± 4.22	0.945 ± 0.0305
ZD 622		5.93 ± 0.12	1.99 ± 0.11	1.66 ± 0.06	0.022 ± 0.0046
	Cu 200 μM	16.83 ± 0.81	16.74 ± 0.69	65.50 ± 1.98	0.882 ± 0.0160
	Cr 200 μM	25.44 ± 2.11	28.67 ± 1.13	96.61 ± 3.14	1.127 ± 0.0410
	Cu 200 μM + Cr 200 μM	35.26 ± 1.85	46.96 ± 2.07	145.53 ± 4.89	1.384 ± 0.0190

Data are the means of three replicates (mean ± SD). Values followed by different letters indicate significant differences followed by Duncan’s multiple range test ( < 0.05) for each cultivar at different metal treatments.