The Plant Proteinase Inhibitor CrataBL Plays a Role in Controlling Asthma Response in Mice

Figure 2

Purified CrataBL detected by HPLC and mass spectrometry. (a) Protein fraction was chromatographed on a C18 column eluted with a linear gradient (5–100%) of 90% acetonitrile in 0.1% TFA in Milli-Q water (solvent B) (t = 0.1 min, 5% B; t = 5 min, 5% B; t = 30 min, 40% B; t = 50 min, 50% B; t = 60 min, 100% B; t = 65–68 min, 0% B) at the flow rate of 0.7 mL/min. (b) MALDI-TOF mass spectrum of CrataBL.

(a)

(b)