Polymorphisms in the Chicken Growth Differentiation Factor 9 Gene Associated with Reproductive Traits

Table 1

Primer pairs used to screen the GDF9 gene for polymorphisms.

Primer pairs name	Primer sequences (5′–3′)	Binding regions	Product size (bp)	Annealing; Tm (°C)	Chr. position
P1	F: GAAGCCGTAAGATGTGAA	Partial promoter region and exon 1, 5′UTR	701	51.5	17156010–17156720
	R: GGAAGAAAGCCAGTGAAT
P2	F: CCTGAGAAGCAGCGTTTG	Exon 1, partial intron 1	796	58.7	17156430–17157225
	R: CAGCAGCCTCCACATTTT
P3	F: ATTGGTTTCTTCTGCTGCTT	Exon 2, partial intron 1	882	58	17157996–17158877
	R: CATAACGGTGCCCGACTA
P4	F: AACACGCAGGGCAAAAGG	Exon 2	971	54.2	17158631–17159601
	R: AAGTCCCGACCAAAGCAG
P5	F: TCTGGGAAAAGAGGAAAG	Partial 3′UTR	711	46	17159411–17160111
	R: GTCTGAAATGGGTTGGTG