Comparative Hemolymph Proteomic and Enzymatic Analyses of Two Strains of Rhipicephalus (Boophilus) microplus Ticks Resistant and Susceptible to Ixodicides

Figure 1

HPLC chromatography profiles of susceptible MJ (a) and resistant SA tick strains (b). At higher concentrations of ACN (acetonitrile), multiple peaks eluted in susceptible strain MJ ((a), upper), while at low concentrations of ACN, peaks were observed in resistant strain SA ((b), upper). The oxidizing activity exhibited through L-DOPA oxidation is depicted below the chromatograms ((a) and (b), lower); the graph exhibits an increase of oxidative activity approximately at 10 min retention time in the susceptible MJ when compared to the resistant SA; additionally, the resistant strain SA exhibits several peaks of oxidative activity which are not present in the susceptible strain MJ during the 20 to 30 min retention time.

(a)

(b)