Comparative Hemolymph Proteomic and Enzymatic Analyses of Two Strains of Rhipicephalus (Boophilus) microplus Ticks Resistant and Susceptible to Ixodicides

Figure 2

Phosphatase and catalase activity gels of hemolymph proteins of susceptible MJ and resistant SA tick strains. (a) In phosphatase zymogram, two protein bands in hemolymph of resistant strain SA were observed while only one protein band was observed in susceptible strain MJ. (b) In catalase zymogram, the protein band patterns of resistant strain SA and susceptible strain MJ hemolymph are different. In resistant strain SA, seven protein bands with catalase activity were observed and only three of these bands were observed in susceptible strain MJ. Arrows indicate protein band position. Larvae hemolymphs of resistant SA and susceptible MJ strains were used as a known positive control of these activities.

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