A Novel Fragment Derived from Laminin-411 Facilitates Proliferation and Differentiation of Odontoblast-Like Cells

Figure 5

Gene expression level of osteo/odontogenic markers and integrins was quantitatively evaluated by real-time RT-PCR after total seven days of in vitro culture of MDPC-23 cells, with three days in mineralization medium. Significant enhanced gene expression levels in MDPC-23 cells growing on iMatrix-411-coated non-PS or TCPS could be detected for OCN (a) (-fold increase in iMatrix-411 modified Non-PS), BSP (b) (-fold increase in iMatrix-411 modified Non-PS), OPN (c) (-fold increase in iMatrix-411 modified Non-PS), ALP (d) (-fold increase in iMatrix-411 modified Non-PS), DMP-1 (e) (-fold increase in iMatrix-411 modified Non-PS), DSPP (f) (-fold increase in iMatrix-411 modified Non-PS), Runx-2 (g) (-fold increase in iMatrix-411 modified Non-PS); regarding integrins, except for ITGA1 (h) (-fold increase in iMatrix-411 modified Non-PS), the other six types of integrin were only mildly enhanced or downregulated by iMatrix-411. Dotted line in each panel divides the data into Non-PS (left) and TCPS groups (right) (, , post hoc Tukey’s HSD test).

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