Negative-Pressure Wound Therapy in a Pseudomonas aeruginosa Infection Model

Figure 11

Fluorescence light microscopy of the NPWT-treated and control P. aeruginosa-infected wounds counterstained with ConA and DAPI at day 2. (a–c) GFP-labeled P. aeruginosa (green) in the NPWT group distributed over the wound (blue) with a sparse glycocalyx matrix, although large aggregates of P. aeruginosa are visible. (d–f) GFP-labeled P. aeruginosa (green) formed a mature biofilm on the control wound surface (blue), showing a complex structure with a large amount of glycocalyx (red) around the bacteria. Scale bar = 100 μm. NPWT, negative-pressure wound therapy; ConA, concanavalin A; DAPI, 4′,6-diamidino-2-phenylindole; GFP, green fluorescent protein.

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