Inhibition of Acetylcholinesterase and Butyrylcholinesterase by a Plant Secondary Metabolite Boldine

Table 3

Found facts about boldine and cholinesterases.

Enzyme	Inhibition mechanism	IC50 (μmol/l) by spectrophotometry	(kcal/mol) in silico	Anionic subsite interaction in silico	Peripheral anionic subsite interaction in silico
AChE	Noncompetitive	372.30	−7.6 (a. site); −6.2 (p. site)	H-bond: Asp 74; π-π: Trp 86	H-bond: Ser 293; π-π: Trp 286
BChE		321.85	−9.7	H-bond: Thr 120, Glu 197; π-π: Trp 82	—