Autophagy Is Independent of the Chondroprotection Induced by Platelet-Rich Plasma Releasate

Figure 2

Effect of PRP on normal chondrocytes or IL-1-treated chondrocytes. The chondrocytes were cocultured with either 2% or 10% L-PRP or P-PRP for 24 or 96 h. (a, c) The expression of cartilage-related genes (Aggrecan, COL2, ADAMTS5, and MMP13) at the mRNA level in chondrocytes treated without (a) or with IL-1β (c). (b, d) Western blot analysis for autophagy-related proteins: LC3 II/LC3 I, Atg5, and Beclin 1. represents significant difference versus control group; # represents significant difference versus IL-1β group.

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