Autophagy Is Independent of the Chondroprotection Induced by Platelet-Rich Plasma Releasate

Figure 3

Effect of PRP on the autophagy flux of normal chondrocytes or IL-1-treated chondrocytes. (a, b, and c) Transmission electron microscopy (TEM) analysis the ultrastructure of control (a), releasate of 10% L-PRP-treated (b) and 10% P-PRP-treated (c) chondrocytes. Chondrocytes were transfected with mRFP-GFP-LC3 to follow autophagic flux, and then they were treated with or without IL-1β and exposed to releasate of 10% L-PRP or 10% P-PRP. (d, e) Confocal images of labeled autophagosomes (yellow) and autolysosomes (red). The number of autophagosomes was quantified (f), and the number of autolysosomes was quantified (g). Data are represented by means ± standard deviation.

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